Fig. 3

Increase in the relative abundance of immunostainable alpha cells in hProC(A7)Y- CpepGFP mice with 1 or 2 functional Ins2 alleles deleted, or in authentic Akita mice. a Paraffin sections of pancreata from mice with the genotypes indicated, were immunostained with anti-insulin (blue) and anti-glucagon (red). Scale bar: 20 μm. b From confocal microscope images, a blinded reader scored the number of glucagon-positive and insulin- positive cells. With ~1000 immunostainable cells per mouse, the fraction of immunostainable cells that were glucagon-positive (called “Alpha”) was quantitated for each mouse (shown as individual points in scatter plot with mean ± SE; *: p < 0.05. As noted in the Results, we did not observe double hormone-positive cells. c Paraffin sections of pancreata from C57Bl/6 control or Akita diabetic (male) mice were immunostained as in panel A, with representative images shown. d From control and Akita diabetic mice, the fraction of immunostainable cells that were glucagon-positive (called “Alpha”) was quantitated and presented as a scatter plot as in panel B, with mean ± SE; *: p < 0.05