Skip to main content
Fig. 4 | Clinical Diabetes and Endocrinology

Fig. 4

From: Interpretation of common endocrine laboratory tests: technical pitfalls, their mechanisms and practical considerations

Fig. 4

Illustration of human anti-mouse antibodies (HAMA) interference with immunoassay. The left panel illustrates the non-competitive “sandwich” immunoassay without the presence of HAMA in the sample (see Fig. 2). The right panel illustrates the mechanism of the HAMA interference with immunoassay. a The sample containing the studied hormone and the HAMA is added to the test tube which contains both capture and signal antibodies. b In addition to the correct formation of “sandwiches” (capture antibody- hormone-signal antibody), the HAMA forms a bridge between the capture antibody and the signal antibody forming antibody-HAMA-antibody “sandwiches”. As a result, more signal will be measured, and thus, false elevation of the studied hormone will be reported

Back to article page